After your eggs (oocyte) are retrieved and brought to the laboratory, we select the most natural way of treatment. Oocytes surrounded by cells called granulocytes are removed from those cells via a special method and made ready for ICSI (microinjection).
Then, by preparing the sperm of the male partner, physiological ICSI is processed at the right time. It is common to use PVP (Ployvinylpyrrolidone), which is a synthetic polymer, in ICSI to slow down and manipulate sperms easily. PVP helps to place the sperm into the egg easily, however, it cannot be metabolised by the egg and cannot be thrown out of the cell. It remains intact in the egg. PVP especially affects egg quality/health negatively in patients whose eggs are not in very good condition. Research has shown negative effects of PVP, especially, on the chromatin structure and the DNA of the sperm. Its effects on the embryo after being used during ICSI are still being researched. Also, research to find alternatives to PVP is still going on. Today, there are two ways of conducting ICSI without using PVP. One is to use hyaluronic acid which is a natural substance already present in granulocytes, and the other is to use neither. This type of ICSI method where PVP is not used is called physiological ICSI.
We prefer "Physiological ICSI" especially on patients whose egg and sperm quality is not good enough in order to improve the health and quality of the embryo.